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(A) Surface preparation of 8-well chambered slides: wells are sequentially coated with biotin-BSA, NeutrAvidin, and either <t>anti-CD16</t> or HER2 protein (for experimental and control conditions). Protein incubations are performed on a see-saw rocking shaker. Each coating step is followed by DPBS rinses. Positive and negative controls are included to assess NK cell activity as well as nonspecific binding to the surface. After coating, wells are filled with RPMI supplemented medium and incubated for 15 min at 37 °C. (B) NK cell preparation and live-cell imaging: primary human NK cells are isolated and characterized by flow cytometry as described in Gonzàlez et al. [12]. Cells are pre-incubated with bispecific antibodies (bsAb) (concentration 0.14–14,000 pM) before seeding onto HER2. BsAb is not added to the control well with an anti-CD16-coated surface. Live-cell RICM imaging is performed immediately after cell addition at 37 °C, capturing time-lapse sequences from multiple positions over a 10-min period.
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https://www.bioz.com/result/anti human cd16 fitc antibody/product/Miltenyi Biotec
Average 95 stars, based on 1 article reviews
anti human cd16 fitc antibody - by Bioz Stars, 2026-03
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igg1 pe cd16 miltenyi biotec  (Miltenyi Biotec)
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(A) Surface preparation of 8-well chambered slides: wells are sequentially coated with biotin-BSA, NeutrAvidin, and either <t>anti-CD16</t> or HER2 protein (for experimental and control conditions). Protein incubations are performed on a see-saw rocking shaker. Each coating step is followed by DPBS rinses. Positive and negative controls are included to assess NK cell activity as well as nonspecific binding to the surface. After coating, wells are filled with RPMI supplemented medium and incubated for 15 min at 37 °C. (B) NK cell preparation and live-cell imaging: primary human NK cells are isolated and characterized by flow cytometry as described in Gonzàlez et al. [12]. Cells are pre-incubated with bispecific antibodies (bsAb) (concentration 0.14–14,000 pM) before seeding onto HER2. BsAb is not added to the control well with an anti-CD16-coated surface. Live-cell RICM imaging is performed immediately after cell addition at 37 °C, capturing time-lapse sequences from multiple positions over a 10-min period.
Igg1 Pe Cd16 Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/igg1 pe cd16 miltenyi biotec/product/Miltenyi Biotec
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igg1 pe cd16 miltenyi biotec - by Bioz Stars, 2026-03
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(A) Surface preparation of 8-well chambered slides: wells are sequentially coated with biotin-BSA, NeutrAvidin, and either anti-CD16 or HER2 protein (for experimental and control conditions). Protein incubations are performed on a see-saw rocking shaker. Each coating step is followed by DPBS rinses. Positive and negative controls are included to assess NK cell activity as well as nonspecific binding to the surface. After coating, wells are filled with RPMI supplemented medium and incubated for 15 min at 37 °C. (B) NK cell preparation and live-cell imaging: primary human NK cells are isolated and characterized by flow cytometry as described in Gonzàlez et al. [12]. Cells are pre-incubated with bispecific antibodies (bsAb) (concentration 0.14–14,000 pM) before seeding onto HER2. BsAb is not added to the control well with an anti-CD16-coated surface. Live-cell RICM imaging is performed immediately after cell addition at 37 °C, capturing time-lapse sequences from multiple positions over a 10-min period.

Journal: Bio-protocol

Article Title: Quantitative Microscopy for Cell–Surface and Cell–Cell Interactions in Immunology

doi: 10.21769/BioProtoc.5427

Figure Lengend Snippet: (A) Surface preparation of 8-well chambered slides: wells are sequentially coated with biotin-BSA, NeutrAvidin, and either anti-CD16 or HER2 protein (for experimental and control conditions). Protein incubations are performed on a see-saw rocking shaker. Each coating step is followed by DPBS rinses. Positive and negative controls are included to assess NK cell activity as well as nonspecific binding to the surface. After coating, wells are filled with RPMI supplemented medium and incubated for 15 min at 37 °C. (B) NK cell preparation and live-cell imaging: primary human NK cells are isolated and characterized by flow cytometry as described in Gonzàlez et al. [12]. Cells are pre-incubated with bispecific antibodies (bsAb) (concentration 0.14–14,000 pM) before seeding onto HER2. BsAb is not added to the control well with an anti-CD16-coated surface. Live-cell RICM imaging is performed immediately after cell addition at 37 °C, capturing time-lapse sequences from multiple positions over a 10-min period.

Article Snippet: Anti-human CD16-FITC antibody (Miltenyi Biotec, catalog number: 130-091-244) 8.

Techniques: Control, Activity Assay, Binding Assay, Incubation, Live Cell Imaging, Isolation, Flow Cytometry, Concentration Assay, Imaging